The Department of Biotechnology, in cooperation with the Continuing Education Unit at the College of Science, University of Baghdad, organized a lecture titled.” Three-Dimensional Cell Engineering “. The lecture aimed to shed light on the specialization of genetic engineering, which is considered one of the best models that can simulate the development of living organisms in the laboratory. It described three-dimensional cultivation systems of natural cancer cells that can be used in preclinical tumor virus treatments. Dr. Marwa Ibrahim Salman presented the lecture, which included an overview of the development of a three-dimensional cultivation system in the laboratory. This involved using two types of breast cancer cell lines to demonstrate different receptor states grown through the use of adipose tissue-derived intermediate stem cells. The importance of arranging cells in a floating tissue culture plate was emphasized to allow the formation of spheroids bodies. These spheroid bodies were then collected and transferred to a scaffold plate using three-dimensional cultivation systems to evaluate the treatment with the Newcastle disease virus strain AMHA1. The lecture also discussed monitoring anti-tumor activity using a tracking system for the Newcastle disease virus (NDV) called fluorescent linker PKH67. The results concluded that the NDV strain AMHA1 replicates sufficiently and has an anti-tumor effect in cancer through three-dimensional natural cell cultivation systems, indicating effective clinical outcomes.

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